Biosynthesis of Porphyrinogens in Etiolated Euglena gracilis Z. II. Identification of a Regulatory Pteridine
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چکیده
A low molecular weight compound which stimulates the synthesis of porphyrinogens from PBG was isolated and purified from etiolated Euglena gracilis Z. Absorption and fluorescence excitation and emission spectra, as well as the molecular weight, revealed its structure as an unconjugated pteridine. FTIR bands and 'H NMR signals of the purified compound were identical with those of an authentic sample of 6-biopterin. The structural formula of this compound is given in Fig. 1.
منابع مشابه
Biosynthesis of Porphyrinogens in Etiolated Euglena gracilis Z. I. Isolation and Purification of an Endogenous Factor Stimulating the Formation of Porphyrinogens
A low molecular weight, heat-stable factor has been purified from Euglena gracilis supernatant fraction by employing gel filtration, cation and anion exchange and paper chromatography. This endogenous compound stimulates porphobilinogenase (PBG-ase) (EC 4.3.1.8) activity, an enzyme of the porphyrin biosynthetic pathway. 1CT M folic acid and ICI" M 6-biopterin produced a significant activation, ...
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Fachbereich Biologie/Botanik, Philipps-Universität Marburg. D-3550 Marburg. Bundesrepublik Deutschland Z. Naturforsch. 44c, 81 — 84 (1989); received September 26. 1988 Porphyrinogens. Euglena gracilis Z ., Porphyrin Biosynthesis. Porphobilinogenase. Euglena Chloroplasts Lysed cells as well as different fractions of isolated, disrupted chloroplasts from light grown Euglena gracilis were tested f...
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تاریخ انتشار 2012